Embryological procedures

ICSI, PICSI, AH, embryo cultivation, prolonged embryo cultivation, embryo monitoring, frozen embryo transfer - FET

In vitro fertilization - IVF

The standard IVF means procedure when the suspension of processed sperms and cultivation medium is added to the retrieved eggs placed on special cultivation dishes.

Intracytoplasmic sperm injection - ICSI

ICSI - Intracytoplasmatická injekce spermieIntracytoplasmic sperm injection (ICSI)The principle of this method is direct injection of a single sperm into the cytoplasm of a mature oocyte. One sperm is selected, immobilized, aspirated by microinjection pipette and inserted into the egg. The size of the sperm head is only 5 micrometers – this procedure requires an experienced embryologist and a special micromanipulator.

The indications of ICSI include:

  1. low sperm count and motility
  2. surgically retrieved sperms
  3. abnormality of the zona pellucida (= the surface of the egg)
  4. fertilization failure in previous treatment

ICSI reduce the risk of canceling the cycle due to absence of fertilization.

Intracytoplasmic injection of preselected sperm - PICSI

PICSI - Intracytoplasmatická injekce preselektované spermieThe principle is binding of the best mature sperm to the hyaluronan - a naturally occurring substance found in all human cells, including the layer surrounding the oocyte. These bound sperms are then used for ICSI. PICSI-selected sperm are more mature and can improve the pregnancy rate.

 

 

Assisted hatching (AH)

AH - asistovaný hatchingAssisted hatching (AH)Assisted hatching is a procedure in which a small hole is made in the zona pellucida, using a special micropipette and micromanipulator. This hole facilitates zona hatching - the blastocyst gets rid of the surrounding zona pellucida to be able to implant in the uterus. We recommend assisted hatching in cases with history of previous implantation failure, age over 35 years or thick zona pellucida.

 

 

 

 

 

 


Basic embryo cultivation

Kultivace embryíKultivace embryíBasic cultivation lasts 48 hours and results usually in 2 or 4 cells embryos.

Prolonged embryo cultivation

Prolonged (or extended) cultivation lasts usually 72 – 120 hours and best embryos can develop in blastocyst. This procedure requires a special medium. The advantages are selection of more viable embryos and better receptivity of the endometrium.

Continuous embryo monitoring – PrimoVision
(Noninvasive time-lapse embryo imaging)

This procedure provides us detailed information about the embryonic cells division during cultivation. The system is computer controlled and the monitoring (one record every 15 minutes) takes place inside the incubator without any disturbances. Digital records analysis helps us select more confidently the embryo with the best developmental abilities. Primo Vision provides quantitative data on embryo development and this objective information improves our ability to select the most viable embryos for transfer or freezing.

Monitorování vývoje embryí – PRIMOVISION systém   Monitorování vývoje embryí – PRIMOVISION systém


Embryo freezing

KryokonzervaceEmbryos that are not transferred as fresh after embryo cultuvation can be frozen and stored in the liquid nitrogen for several years. We freeze embryos successfully since 1992!
Vitrification method (rapid cooling technique) is used for freezing.

Oocyte freezing

NOocytes that are not fertilized after egg collection can be frozen and stored in the liquid nitrogen for several years. We started freezing oocytes successfully in 2011! Vitrification method (rapid cooling technique) is used for freezing.

Frozen embryo transfer (FET)

KET - přenos embryí po rozmraženíIf you’ve had embryos frozen during a previous cycle, we can thaw them and transfer to your womb. Special medication is usually used to prepare your womb (endometrium) to receive the embryos. We monitor the uterus to assess the right time for the transfer with the highest chance of achieving a pregnancy.





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